【摘要】 我们建立的K562/ADM耐药细胞株,在ADM浓度为2.4μg/m1(4.46μM)中已稳定培养3.5个月,传了30—35代,K562/ADM亦具有多药耐受件(Multidrug Resistance,MDR)的特点,对ADM、VCR、AT—1258和DDP的耐受性分别为K562的114.7、94.0、13.3和7.4倍,但对5—FU不产生交叉耐药。K562和K562/ADM的倍增时间分别为19.2h和52.8h,集落生成率分别为37.5％和11.1％,K562染色体数为34—68,中位数为56;K562/MDM染色体数为32—90,中位数为50,K562/ADM可做为耐药机理和克服耐药措施研究的极好模型。更多还原

【Abstract】 A K 562 cell line resistant to ADM was established by cultivation in increasing concentration of ADM. It has been growing for 30-35 generation in 3.5 months in a medium containing 2.4μg/ml (4.46μM). It was characterized with multidrug resistance(MDR), and its resistance to ADM, VCR, AT-1258 and DDP, were 114.7, 94.0, 13.3, and 7.4 time as compared with normal K562 cell line, but with no cross-resistance to 5-Fu. The doubling times of K562 and K562/ADM were 19.2 hr. and 52.8 hr., and rates of colony formation of them, 37.5% and 11.1% respectively Numbers of chromosome and median of K562 were 34-68 and 56 respectively, and K562/ADM, 32-90 and 50. K562 cell line resistant to ADM may be used as a model for the study of mechanism and reverse method of anticancer-drug resistance.更多还原