【摘要】 <正> P53基因定位于人17号染色体短臂,正常的P53基因作为一种细胞增殖的抑制因子在正常的细胞中调节生长和分化,但若基因突变或丢失,就使这种调节作用丧失。目前,巳有大量报道,在人脑、乳房、肺、骨、结肠、直肠的恶性肿瘤里,均发生P53基因的突变和野生型P53等位基因经常伴随着丢失。这些研究提示正常的(野生型的)P53产物可能起着抑制瘤生长的作用,而突变或丢失,或两者兼更多还原

【Abstract】 Deletions of P53 gene occurred commonly in human bepatic carcinomas,suggestingthat the wild-type gene may act as a suppressor of hepatic carcinoma cell growth. Totest this hypothesis, wild-type human P53 genes were transfected into human hepato-cellular carcinoma cell line PLC/PRF/5 .The transfected cell showed tenfold less colony-forming efficiency than the control group. The state of P53 gene in those colonies thatdid form after wild-type transfection needs further study.更多还原