【摘要】 继代半年的玉米自交系"黄早4"的幼胚产生的胚性愈伤组织,转入加有不同浓度(5、10、15、20、25、30g/L)NaCl 的筛选培养基上培养,经过三代筛选,获得耐盐愈伤组织。NaCl浓度显著影响愈伤组织的生长和存活率。培养基中 NaCl 为1 0g/L 时,筛选出的愈伤组织生长较好,可在分化培养基上再生植株。再生植株在加有 NaCl 10g/L 的壮苗培养基上生根长大。耐盐愈伤组织在加有10g/L NaCl 的培养基上,游离氨基酸增加18%,其中,脯氨酸增加87.3%;Na+增加661.9%,K+增加25.5%,Ca++显著减少;细胞及细胞器形态结构正常,代谢活动旺盛。未经筛选的愈伤组织(对照)在加有10g/L NaCl 的培养基上,绝大多数细胞死亡或处在类坏死状态。耐盐愈伤组织再生的植株及其子代,虽生长在非盐渍化的土壤中,其后代仍表现出耐盐性,并在耐盐程度上出现分离。其中一株再生植株的后代在10g/L NaCl 的溶液中能正常萌发和生长。我们获得的耐盐愈伤组织及再生植株是耐盐变异体。更多还原

【Abstract】 The embryonic calli produced from immature embryos of inbred "Huangzhao-4" of maize, that had been maintained for half a year, were transferred to media supplemented with different NaCl concentrations （5, 10, 15, 20, 25, 30g/L） for callus selection. NaCl tolerant calli were established through three generations of selections. The growth and frequency of survival calli were affected significantly by NaCl concentration. The proliferetion of NaCl-to-lerant calli was relatively good on medium containing of 10g/L NaCl. From these calli, plant lets could be produced on differentiation medium. On medium supplemented with 10g/L of NaCl the plantlets could normally grow to transplantation. In NaCl-tolerant calli cultured on medium containing 10g/L of NaCl, the contents of free amino acids, free proline, Na⁺, K⁺ were 18.0%,87.3%,661.9%,25.5% respectively higher than those in un-selected calli grown on subculture medium, but Ca²⁺ content decreased significantly. On medium containing 10g/L of NaCl, cells and their organelles in NaCl-tolerant calli had normal morphology and structure, and vigorous metabolism, but in un-selected calli, the majority of cells turned to wards dying. Although tolerant plants regenerated and their filial ones had grown in non-salted soil, their progenies retained the property tolerance, but showed segregation of the degrees of tolerance. In 10g/L NaCl solution, the seeds of progenies from one plant regenerated could germinate normally, and grow into healthy seedlings. Therefore, the NaCl-tolerant calli and plantlets that we have obtained NaCl-tolerant variants.更多还原