【摘要】 本研究从我国的6个甜椒(Capsicum frutescens)栽培品种中筛选出"双丰"、"中椒二号","中椒三号"3个再生能力较强的基因型,以苗龄10-16天的子叶为外植体,经过芽诱导、芽伸长、根诱导、移入土壤四个步骤的离体培养,已获得能正常开花结实的再生植株。所有培养基以MS为基本培养基,附加不同种类不同浓度的植物激素。最适的芽诱导培养基为MS+4-6mg/L BA+0.5mg/L IAA,芽诱导率可达100%。诱导出的芽转入MS+2mg/L zeatin或2mg/L BA+1-2mg/L GA;的培养基上,可使芽伸长,伸长率为35%左右。已伸长的芽在生根培养基MS或MS+0.1-0.5mg/L NAA上生根后,转入土壤成为正常植株。以子叶为外植体,用叶盘法进行甜椒的基因转化工作,筛选出侵染性强的菌株C58和GV3111,并检测到GUS基因在甜椒子叶上的短期表达。在含50mg/L卡那霉素培养基上已获得抗性芽,进一步检测工作以及转移抗烟草花叶病毒和黄瓜花叶病毒基因的工作正在进行。更多还原

【Abstract】 Three strains of sweet pepper, (Capsicum frutescens) "Shuang Feng", "Zhong Jiao No. 2" and "Zhong Jiao No.3" were screened out of six Chinese cultivars for their high capacity of regeneration. The normal flowering and fettile regenerated plants have been obtained from cotyledons of seedlings from 10 to 16 days old by a four-step culture procedure short induction, shoot elongation, rooting of excised shoot and transplanting into soil. MS was the basal medium in all steps, supplemented with different kind and different concentration of phyto-homores. Optimal shoot ioduction medium is MS+ 4-6mg/LBA + 0.5mg/LIAA which gives rise a shoot regeneration frequency of 100%. 35% of the induced shoots elongated on the medium of MS + 2mg/L Zeatin or 2mg/LBA + 1-3mg/L GA, and subsequently rooted on MS medium or in addition of 0.1-0.5mg/LNAA. The regenerants were trans￡lanted into soil and developed into normal plants. In the transformation of sweet pepper using the leaf disc method, two kinds of wild type Agrobacterium tumefaciens, C58 and GV3111, have been screened out in regard to their high infection capacity. The transient expression of GUS gene was detected and Ka- namycin-resistant shoots from infected cotyledons have been obtained. Further assay and tran- sfering the TMV-resistant and CMV-resistant genes into sweet pepper are in progress.更多还原