【摘要】 从感病的烟草叶片中分离纯化了马铃薯X病毒(PVX)。用人工合成的寡核苷酸作为引物,合成了cDNA并克隆到Bluescript载体上。通过分子杂交鉴定、限制性内切酶图谱分析和DNA全序列测定,证明克隆了完整的马铃薯X病毒的外壳蛋白基因。测定的cDNA序列与国外发表的PVX外壳蛋白基因的序列相比较,发现有很高的同源率。并将外壳蛋白基因转入马铃薯中,获得了在卡那选择培养基上生长的马铃薯幼苗。更多还原

【Abstract】 Potato virus X(PVX) was isolated and purified from infected tobacco leaves. The cDNA was synthesized with synthetic oligonucleotide as a primer and cloned into bluescript vector. The gene encoding coat protein of PVX was cloned with restriction enzyme analysis and DNA hybridization. The full length of DNA sequence of the gene was determined and compared with sequence reported before. It was found that the gene cloned from PVX infected in China has high homology in DNA sequence to that reported abroad. We transformed the coat protein gene to potato plants and obtained potato shoots on selective medium.更多还原