【摘要】 选用对吸胀冷害敏感的大豆(Glycine max Merrill)品种“黑河3号”为材料,从生物膜的角度,利用系列生理生化的指标论证吸湿-回干处理对大豆种子再水合过程中膜修补的关系。吸湿-回干处理可以明显提高种子抗吸胀冷害的能力,表现在种子活力的提高与种子渗漏物的减少,同时,K~+/Na~+比降低、K~+-Na~+-ATP 酶活性提高,表明在细胞质膜上的透性调控机制的运转效应得到了增强与维持。超氧化物歧化酶(SOD)活性、过氧化氢酶和过氧化物酶活性增强,挥发性醛类释放量减少,显示出细胞抗过氧化能力的增强。根据系列生理生化指标和吸水曲线的特性,作者提出了吸湿-回干诱导膜系统“预修补”的模式,确认物理与生化修补这两个修补过程的效应在“回干”后能得以保存。更多还原

【Abstract】 The soybean(Glycine max Merrill)cv.“Heihe No.3”which is sensitive to imbibition- al chilling injury was used as experimental material.From the point of cell membranes and by means of physiological tests,the relation of hydration-dehydration to membrane repairation during rehydration in seeds was discussed,and a new point of view on the acting mechanisms of hydration-dehydration was approached.The resistance of seed to imbibitional chilling inju- ry was obviously enhanced,its apparent efficiency displayed the enhance of seed igor,redu- ction of leakage,especially the K~+/Na~+ ratio,increasing the K~+-Na~+-ATPase activity.The facts indicate that the function of cytoplasmic membrane was enhanced and maintained nor- mally.In the meantime,the activities of SOD,catalase and peroxidase were all enhanced,and the production of volatile aldehydes were decreased.It shows that the function of cell anti- autoxidation system was greatly improved. Based on a series of physiological and biochemical index mentioned above and the com- parison of water-absorbed curves,a postulated model of membrane system induced by hydra- tion-dehydration treatment is given.It is considered that the effects of the two processes,i,e. physical membrane repairation as well as the physiological and biochemical repairation,will be persisted in the redehydrated seeds更多还原