【摘要】 本文分析研究了中国美利奴羊新疆型与军垦型羊毛的角蛋白组成。通过尿素-变性凝胶电泳、SDS-凝胶电泳和双向电泳等的实验结果表明:中国美利奴羊新疆型和军垦型羊毛的角蛋白分别有16-21个主要组分,可分为低硫蛋白、高硫蛋白和高甘氨酸/酪氨酸蛋白三族,其表现分子量力59600-8200 Dalton,通过进一步对电泳凝胶光密度扫描测得,中国美利奴羊毛的角蛋白组成含量与澳州美利奴羊毛相近,与新疆细毛羊和军垦细毛羊羊毛比较,其高硫蛋白含量有明显增加,由于遗传因素引起的蛋白质组分的差异也主要表现于高硫蛋白中。更多还原

【Abstract】 Sheep wool (Xinjiang type and Junken type) have been investigated. The procedures include extracting soluble keratin proteins from wool by means of reductant; examing keratin components and determing molecular weights by urea-containing denatural polyacrylamide gel electrophoresis (PAGE), SDS-PAGE and two-dimensional PAGE. It has shown that the Chinese Merino Sheep wool contains 16-21 major proteins which could be classified into the Low-sulphur, High-sulphur and High-glycine/tyrosine protein groups-The range in apparent molecular weights for these proteins is 59600-8200 Dalton.Compared with Xinjiang Fine Sheep wool and Junken Fine Sheep wool, further, by the densitometric scanning of electrophoretic patterns, the wool samples from Chinese Merino Sheep showed a significant increase in High-sulphur protein content and was similar to Australian Merino wool. The genetic variation has also been observed in Highsulphur protein fractions.更多还原