【摘要】 本试验研究了诱发结晶温度、缓慢降温速度、冷冻速度、解冻温度和冷冻前短暂体外培养对半胚冷冻效果的影响,证明在下述条件下对琼脂包被半胚进行冷冻和解冻可显著提高山羊半胚冷冻效果:①在-7℃诱发结晶;②以约0.005℃/分的速度缓慢降温15分钟;③以0.3℃/分的速度降温至-35℃投入液氮;④在30-35℃的水浴中解冻;⑤在冷冻前对半胚进行2小时短暂体外培养。将按上述条件冷冻-解冻的12枚半胚移植于6只受体,结果有4只妊娠(66.7%),共产半胚羔5只(41.7%),其中包括1对异龄同卵双生羔和1对同龄同卵双生羔(33.3%)。更多还原

【Abstract】 Effects of seeding temperature, slow cooling rate, freezing rate before depositing in liquid nitrogen, thawing temperature and brief culture in vitro before freezing on freezing of demi-embryos were studied. The results show that freezing of demi-embryos embedded with agar under the following conditions can improve survival rate of frozenthawed demi-embryos: (1) seeding at-7℃; (2) slow cooling at about 0.05℃/min for 15 rain; (3) continuous cooling at 0.3℃/min to-35℃ at which demi-embryos were plunged into liquid nitrogen; (4) thawing in water at 30-35℃; (5) brief culture in vitro for 2 h before freezing. Twelve demi-embryos were frozen and thawed on the basis of above conditions and then transferred. of 6 recipients, 4 became pregnant (66.7%) and produced 5 demi-embryo kids (41.7%) at delivery, including 2 pairs of monozygotic twins (33.3%), one pair with different ages, another pair with the same age.更多还原