【摘要】 本文对9例HBsAg阳性母亲所生,并于0、1、6月龄时接种乙肝疫苗免疫失败而形成HBV持续感染的小儿,进行外周血单个核细胞(PBMC)中有无HBV感染的研究.应用未标记酶抗酶免疫复合物法(PAP)检测HBsAg和HBcAg,原位核酸杂交检测HBV-DNA;10例抗HBs阳性幼儿作为对照.结果:9例血清HBsAg阳性幼儿的PBMC中有4例HBV-DNA阳性.其中3例HBsAg同时阳性.另有1例HBsAg阳性,但未测到HBV-DNA.HBc Ag均阴性.HBV-DNA分布于胞浆中,阳性细胞数约占细胞总数的1～2%.对照组PBMC中HBV-DNA、HBsAg和HBcAg均阴性.上述结果表明,HBV已感染PBMC,并在其内表达S抗原.更多还原

【Abstract】 This study investigated the HBV infection status in the peripheral blood mononuclear cells (PBMC) of 9 "healthy" babies who were non-responders to 3 routine HB vaccination after their delivery and had become HBsAg positive in their sera since 1 or 3 months of age. Their mothers were all chronic HBsAg carriers with positive HBeAg in their sera. A control group of 10 healthy anti-HBs positive babies was selected after their HB vaccine immunization.The heparinized venous blood was taken and the PBMC were isolated to detect the HBV DNA and HBV antigens in PBMC by PAP method and in situ hybridization technique respectively. HBV DNA was demonstrated in the PBMC of 4 babies, 3 of whom as well as another one were positive in HBsAg. But HBcAg was all negative in the PBMC of this study group. HBsAg was found to be mainly distributed in the cytoplasm of PBMC but rarely on the cell membranes with cap formation. HBV DNA was demonstrated in the cytoplasm of PBMC, which might be considered as free HBV DNA sequences. The proportion of the HBsAg positive cells in PBMC was estimated to be 1～2%. No positive HBV DNA and HBV antigens were detected in the PBMC of any of the controls. The results strongly suggest that HBV does infect and replicates in PBMC, the mechanism of HBV infection in PBMC remains to be studied.更多还原