【摘要】 采用选择性抽提方法结合整装细胞电镜技术（whole mount cell EM）及扫描电镜技术,对单核、巨噬细胞的骨架系统进行了观察;并应用抗肌动蛋白（Anti-actin）抗体和抗α-管蛋白（Anti-αtubulin）单抗结合胶体金免疫电镜技术分别对其微丝、微管进行了定性研究。在此基础上,对不同剂量⁶⁰Coγ射线照射前后单核、巨噬细胞骨架系统的辐射效应进行了观察,发现照射后细胞骨架系统发生了显著的改变。同时,对照观察了秋水仙素、松胞素B对其骨架系统的影响,发现它们之间存在着一些类似之处。因此,我们认为⁶⁰Coγ射线引起的细胞骨架系统改变可能与微管、微丝的聚合障碍有关。更多还原

【Abstract】 The whole mount cell electron microscopy in combination with selective extraction method for preparing cytoskeletal framework was applied. Cytoskeleton prepared by Triton X-100 treatment of mouse peritoneal macrophages and human peripheral blood monocytes appeared in electron microscopy as a highly organized and interconnected three-dimensional matrix of different fibrous elements. Since such cytoskeletons are open membrane-free system, individual fibrous organizations can be identified by specific antibodies. An indirect immuno-gold procedure using monoclonal anti-tubulin or anti-actin antibodies was applied to visualize tubulin-or actin-containing structures. The three-dimensional visualization of Triton X-100 resistant cytoskeletons had been used to demonstrate that different doses of 60Co Y-ray caused a distinctive and reproducible alterations of the cytoskeletons of intact mouse peritoneal macrophages and human peripheral blood monocytes in vitro. The results showed that there were some similar alterations with those caused by cytochalasin B and by colchicine. From these observations and other workers’ studies, It’s likely that 60Co 7-ray irradiation may inhibit cytoplasmic microtubule and microfilament assembling.更多还原