【摘要】 利用同位素标记的抗人活化血小板单克隆抗体,经放射免疫法来评估14例支气管哮喘发作时体内血小板的活化程度,结果为:（1）哮喘发作时血小板α-颗粒膜蛋白（GMP-140）分子数在质膜上表达显著增高（P<0.00）,血浆内GMP-140的分子数也显著升高（P<0.00）,而血小板膜糖蛋白Ⅰ_b及Ⅲ_a的分子数无显著改变（P>0.05）;（2）血浆内血栓素B₂（TXB₂）的浓度呈显著升高,TXB₂与6-酮-PGF₁α的比值显著增高（P<0.001）;（3）血小板α-颗粒释放产物β-血小板球蛋白（β-TG）及血小板因子4（PF₄）的血浆浓度均显著升高;（4）血小板数量明显下降。结果表明哮喘发作时体内血小板的活化程度显著增高,为进一步研究血小板活化在哮喘发病中的意义提供了依据。更多还原

【Abstract】 Radioimmunoassay with specific monoclonal antibody was used to evaluate the platelet activation in 14 cases of acute bronchial asthma. The result showed that the number of molecules of alpha-granule membrane protein (GMP-140) which was exposed on the surface of platelet following secretion significantly increased on the surface of platelet and in plasma, while the number of molecules of glycoprotein(GP) Ib and GPIIIa,did not change significantly; the concentration of thromboxane B2 in plasma was raised, while the concentration of 6-keto-PGFiu was within the normal limits; the concentrations of (3-thromboglobulin(B-TG)and platelet factor 4(PF4)in plasma increased significantly; the number of platelets decreased. These results strongly confirmed that the degree of platelet activation was enhanced during acute asthmatic attack. The significance of platelet activation in the pathogenesis of asthma should be further investigated.更多还原