【摘要】 利用自制的抗人活化血小板α-颗粒膜蛋白（Granule MembraneProtein-140,GMP-140）特异性单克隆抗体SZ-51,经放射免疫法直接定量测定血小板表面GMP-140的分子数及血浆内GMP-140的含量,对临床多种疾病（如急性心肌梗塞、脑血栓形成、糖尿病、支气管哮喘、流行性出血热等）体内血小板活化及其程度进行了评估,并与血浆内花生四烯酸代谢产物TXB₂及Von willebrand因子（vWF）含量比较,证实血小板表面GMP-140分子数的测定优于TXB₂及vWF等的测定。血小板表面GMP-140分子数能敏感地反映血小板的活化程度,是判断血小板活化特异的指标之一,这一方法的建立,将为血栓性疾病的诊断及其它疾病发病机理的进一步探讨提供依据。更多还原

【Abstract】 Using monoclonal antibody (McAb) SZ-51 which is specific for an alpha-granule membrane protein (GMP-140) on the surface of human activated platelets,the platelet GMP-140 expression in fixed whole blood was measured by direct radioimmunoassay and GMP-140 microparticles in plasma was measured by sandwich method. The GMP-140 molecules per platelet or milliliter(mL) were calculated for the following subjects: acute myocardial infarction; cerebro thrombosis; diabetic mellitus; asthma attack ; epidemic hemorrhagic fever etc. By comparing with the concentration of thromboxane B2 (TXB2) and von willebrand factor(vWF) in plasma,it is confirmed that the measurement of GMP-140 molecules is better than that of TXB2 and vWF. It is a sensitive and specific method for evaluating the platelet activation degree in vivo. The establishment of this method will be useful to diagnosing the thrombotic disorders and studying the pathogenesis of some other diseases.更多还原