【摘要】 试验表明,碘伏对蜡状杆菌芽胞具有较强的渗透力和氧化力,可破坏通透性屏障,使酶活性消失,并导致核心物质漏出,以至死亡。更多还原

【Abstract】 In the study of bactericidal mechanism of iodophor against spore of Bacillus cereus, the spores were exposed to iodophor solution (containing 100 ppmof available iodine) for 5~60 min and changes of their morphology, enzymes, dipicolinic acid (DPA) and DNA release were then observed. After exposure to iodophor, scanning electron microscopic examination of the spores revealed swelling, decrease in refractivity , shrinkage of surface and even disintegration. The changes of spores found under transmission electron microscope were swelling of exosporium, coat and core , decrease in electron density of cortex, break of coat and appearance of large electron--transmission area. Histochemical examination indicated significant decrease or even disappearance of activities of glucose-6-phosphate dehydrogenase, lactic dehydrogenase and alkaline phosphatase. DPA and DNA in the leakage were found increased by colorimetric examination and ultraviolet spectrophotometry respectively. The results indicated that the effects of iodophor on spores of B. cereus are mainly injuring their permeability barrier, destroying the core and inactivating the enzymes, thus killing them.更多还原