【摘要】 本文观察了体外HSV-I感染对人淋巴细胞增殖、分化,产生免疫球蛋白的影响。HSV-I能感染PWM刺激的人扁桃体淋巴细胞,并抑制其增殖和免疫球蛋白（IgG）的产生,感染实验组的IgG产量较对照组明显降低（P<0.005）。HSV-I感染后,上清中可检出一定量的病毒,其滴度为10^3·2—10^5·7TCID50/ml;直接免疫荧光法检出病毒抗原阳性细胞为3％—8.5％。在感染实验系统中加入重组的IFN_a（r-IFN_a）、IFN_γ（r-IFN_γ）和纯化的肿瘤坏死因子（TNF）,观察到r-IFN。和TNF均有一定程度抗病毒作用,部分解除HSV-I对IgG产生的抑制作用。和对照组相比,差异显著（P<0.01）。r-IFN_γ对HSV-I的感染和IgG产生几乎无作用（P>0.05）,但r-IFN_γ对TNF有协同作用:抑制病毒增殖和解除HSV-I对IgG产生的抑制,比单独用TNF组更为显著（P<0.01）。更多还原

【Abstract】 We successfully established a system in vitro that HSV-Ⅰ infected human tonsillar lymphocytes. Tonsillar lymphocytes prestimulated with PWM（10μg/ml） were infected by living HSV-Ⅰ, so as to their proliferation and IgG synthesis were abated significantly（P<0.005）.In the supernatants, the infective titers ofHSV-Ⅰ were about 10^3·2—10^5·7 TCID₅₀/ml,number of viral antigen positive cells about 3％—8％.We found that some lymphokine and monokine can dampen the inhibition of infection of infectious HSV-Ⅰ The effect of purified recombinant IFN-α was stronger than that of IFN-γ（p<0.01）. The purified tumor necrosis factor （TNF）also abated alone the inhibition of HSV-Ⅰ markedly, so that IgG synthesis enhanced more highly than that without adding TNF （P<0.01）. TNF in combination with IFN-γ caused a larger increasing in IgG synthesis than that observed for TNF alone（P<0.01）or IFN-γ alone（P<0.01）. This result showed that the activity of TNF had dramatically synergized with IFN-γ.更多还原