【摘要】 本文在pH 7.40,37℃磷酸盐缓冲体系中,研究了铜（Ⅱ）的去甲斑蝥酸络合物（Cu（Ⅱ）/H₂DCA）存在下,不同因素对抗坏血酸（H₂A）断裂DNA链的影响。实验结果表明,Cu（Ⅱ）/H₂DCA催化抗坏血酸有氧氧化过程中产生的H₂O₂进一步发生Fenton反应,生成的·OH是抗坏血酸断裂DNA链的真正物种。但简单的Fenton反应不能解释本实验的所有结果。更多还原

【Abstract】 Effects of various factors on the DNA- breaking action of ascorbic acid in the presence of Cu（Ⅱ）/demethylcantharic acid complex were studied in phosphate buffer （pH 7.40 ）, at 37.0±0.1℃.It was shown that·OH produced from Fenton reaction of H₂O₂ which can be formed from the oxidation of ascorbic acid was responsible for the DNA- breaking action of ascorbic acid and the simple Fenton reaction cannot explain all the results.更多还原