【摘要】 本文报道应用高效液相色谱法对甘草中三种皂甙类成分甘草酸(glycyrrhizic acid,S-Ⅰ),乌拉甘草皂甙甲(uralsaponin A,S-Ⅲ)和乌拉甘草甙乙(uralsaponin B,S-Ⅱ)进行分离和含量测定。采用ODS柱,乙腈—3%乙酸(47:53)为流动相,248 nm为检测波长,三种皂甙分离良好,可在20 min内完成测定。更多还原

【Abstract】 A new analytical method for the separation and simultaneous determination of three saponins in Licorice root by high performance liquid chromatography has been developed. The saponins, glycyrrhizic acid （S-I）, uralsaponin B （S-Ⅱ） and uralsaponin A （S-Ⅲ） were separated and determined by use of the solvent system CH₃CN—3% HOAc （H₂O）（47: 53） at 248nm, and with gradient increasing flow rate, the operation can be completed in 20min. This method is sensitive and acurate with good reproducibility. The contents of three saponins in Chinese Licorice roots derived from four Glycyrrhiza species were determined.更多还原