【摘要】 本文报道用辣根过氧化物酶标记抗独特型抗体NP₃₀进行Dot-ELISA直接法,检测人血清中的日本血吸虫抗体。急性血吸虫病人粪检阳性符合率88.9％:慢性病人74.1％;正常人的假阳性率为3％。与常规ELISA方法相比,均无显著的差别。提示用酶标记NP30的Dot-ELISA同样可以用于检测日本血吸虫抗体,且方法简便易行,便于现场应用。更多还原

【Abstract】 The present paper reported that a murine IgM monoclonal antibody named Np30, which was proved to be an internal image of a gut associated antigen molecule of S. japonicum in function , was labelled with HRP and used to detect the antibody response of individuals acutely and chronically infected with S. japonicum by means of Dot-ELISA, a direct detection assay. The results showed that the test employed in this study was statistically comparable in effect to conventional EL1SA for the diagnosis of schistosomiasis japonica, but much simpler in operation and easier for the field use.更多还原