【摘要】 应用蛋白质印渍技术(western blot)分析日本血吸虫感染小鼠治疗前后对可溶性虫卵抗原(SEA)的抗体应答变化,结果表明:在治后剖杀无虫或只检获雄虫的小鼠血清,对SEA组分蛋白的识别谱型与相应鼠的治前血清相比,或是显色带明显减少,或是针对雌虫和/或虫卵的部分抗原组分先行消失,尤以分子量分布在95～111KD范围的抗原组分明显;如剖杀时仍检获雌雄合抱虫体的,其识别谱型与治前相同。提示化疗后,宿主血清对雌虫和/或虫卵相关抗原的识别带的消失似可作为化疗效果的考核指标。更多还原

【Abstract】 A comparison in recognition of S.japonicum SEA with sera collected from infected mice before and after treatment with praziquantel was done by western blot technique. It was found that as compared with the antigenic pattern before treatment, the decrease in numeber of recognizable bands or disappearance of those against female adult worms and/or egg antigens, mainly involved those bands ranging from 95KD to 111KD. This change in recognizable antigenic pattern might reflect the effectiveness of chemotherapy because no worms or only male worms could be detected from mice after treatment.更多还原