节点文献

间接血凝试验诊断血吸虫病的研究

STUDIES ON THE DIAGNOSIS OF SCHISTOSOMIASIS WITH IHA

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 吴福东谢治民袁斯姣赵清海郭家钢董长安潘炳荣黄文长周宪民

【Author】 Wu Fudong1, Xie Zhimin1, Yuan Sijiao1, Zhao Qinghai1, Guo Jiagang1, Dong Changan2, Pan Bingrong2, Huang Wenchang2, Zhou Xianmin2,1. Institue of Parasitic Diseases, Jiangxi Province 2. Jiangxi Medical College

【机构】 江西省寄生虫病研究所江西医学院寄生虫学教研室江西医学院寄生虫学教研室

【摘要】 我们制备的间接血凝抗原,其阳性捡出率为95.31%,假阳性率为1.71%,与并殖吸虫病有31.30%的交叉反应,对其它常见的人体寄生虫病没有明显的交叉反应;动物实验结果,阳性反应最早出现为15d,最迟30d;实验兔治疗后1年阴转率为82.35%;血吸虫病人治疗2年阴转率为84.21%;致敏血球置4℃冰箱或室温(5~38℃)保存2年效价一致;IHA重演性好;判别阳性标准滴度以1:10为宜;记录IHA反应结果的时间与温度有关。

【Abstract】 The antigen used in IHA test was produced in our laboratory. The detection rate for confirmed positive sera from schistosome patients was 95.31 %. while false positive rate was 1.71%. The cross-reaction with paragonimiasis was 31.3% but none with other parasitic diseases. Antibody reaction in rabbits appeared from 15th to 30th day after infection. The negative conversion rate in cured rabbits was 82.35% one year after praziquantel therapy and the rate in patients was 84.21% two years after treatment.Lyophilized red blood cells sensitized with antigen maintained their sensitivity after being stored at 4℃or room temperature ( 5-38℃ ) for two years. IHA test with antigen of the same batch was highly reduplicative. It was appropriate to take the titre of 1 : 10 as the criterion for diagnosis and the time for reading the result should be related to temperature.

  • 【文献出处】 中国血吸虫病防治杂志 ,Chinese Journal of Schistosomiasis Control , 编辑部邮箱 ,1991年03期
  • 【被引频次】51
  • 【下载频次】92
节点文献中: 

本文链接的文献网络图示:

本文的引文网络