【摘要】 本文对Klaassen等人介绍的测定肝G6p方法作了一些修改,并对此法的可靠性作了验证。结果表明,此法线性关系好,精密度高,回收实验回收率为102.17%。采用本法作正常大鼠108只,测定肝G6p活性的95%正常值范围为9.93～22.63活性单位。CCl₄染毒后,大鼠肝G6p活性明显下降。血清G6p和无机磷含量无明显改变。由此,作者认为,肝G6p是研究肝毒性的重要指标,而血清G6p测定由于血磷基础水平较高,不宜取代肝G6p测定。更多还原

【Abstract】 The present paper reports on the method of measurement of the liver enzyme glucose -6-phosphatase (G6P) to which some modification was made. The reliability of the method was confirmed by linear relationship analysis test (r=0.998), accuracy test, and recovery test (recovery=102.17%). The normal value of liver G6P was evaluated for 108 Sprague-Dawley rats (9.93-22.63 units).It was shown that the rat liver G6P activity decreased after exposure to carbon tetrachloride (CCl4) but there was no obvious change in serum G6P. It is hypothesized that such a difference between liver and serum might be related to the high level of serum phosphorus.更多还原