【摘要】 本文报道了微量元素硒对培养纯种Wistar大白鼠胎肝细胞核酸及蛋白质合成的影响。观察了肝细胞培养基中不同硒水平对³H-TdR（³H-脱氧胸苷）、¹⁴C-UR(¹⁴C-尿苷)参入率的影响,同时还测定了培养基中白蛋白及甲胎蛋白（AFP）的含量。结果显示:细胞培养基中硒（Se）浓度在0.4～0.6μg/ml范围内使³H-TdR、¹⁴C-UR的参入率、白蛋白及甲胎蛋白含量均明显高于不补硒组。说明硒在这一浓度范围内能促进肝细胞DNA、RNA更新率及蛋白质合成。更多还原

【Abstract】 In order to determine the effect of trace elementselenium on dcoxyribonucleic acid （DNA）, ribonucleicacid （RNA） and protein synthesis in culturing fetal rathepatocytes, the incroporation of ³H-deoxythymidine（³H-TdR） into DNA, ¹⁴C-uridine (¹⁴C--UR) intoRNA by fetal rat hepatocytes cultured inselenium--supplemented culture medium were investigated In addition the amounts of albumin and fetalprotein released from the same hepatocytes into the cul-ture medium were also determined. It was shown thatthe incorporation rate of ³H--TdR into DNA and¹⁴C--UR into RNA by fetal hepatocytes cultured in themedium supplemented with selenium as selenite at arang of Q 4 to Q 6 μg per ml levels were significantlyhigher than those cultured in medium withoutselenium--supplementation, concomitantly the amountsof albumin and--fetal protein released into the sameselenium--supplemented culture medium were alsogreater signicantly than those did in the mediumwithout selenium--supplementaion （as control）. Theresults suggest that selenium supplementation in culturemedium at the levels of Q 4 to Q 6 μg selenium per mlcan promote the synthesis of DNA, RNA and proteinby fetal rat hepatocytes更多还原