【摘要】 从176株细菌中,筛选出苯甲酸1,2-双加氧酶的高活力菌株假单胞菌(Pseudomonas)137。进行了该菌产酶的发酵条件试验。产酶的最适温度为32℃,最适起始pH为6.5—7.0。葡萄糖、麦芽糖和甘油对产酶有明显的抑制作用,苯甲酸钠对产酶有促进作用。氨态氮对菌体生长和产酶是必需的。琥珀酸钠是酶形成的有效诱导物。采用0.1%苯甲酸钠和0.2%琥珀酸钠培养基(pH6.5—7.0),于32℃振荡培养72小时,可获得高活力的苯甲酸.,2-双加氧酶,每克菌体酶活力可达5—8单位。更多还原

【Abstract】 Pseudomonus sp. 137, a high-yielding strain for producing benzoate 1,2-dioxygenase was screened from 176 bacterial strains. The conditions for enzyme production of the strains were examined. The optimal temperature and pH for enzyme formation were 32℃ and pH 6.5——7.0 respectively. Enzyme formation was enhanced by sodium benzoate, and was markedly inhibited by glucose, maltose and glycerol. Ammonium nitrogen sources were essential for cell growth and enzyme production. Sodium succinate was an effective inducer for enzyme formation. When the organism was grown in 0.1% sodium benzoate and 0.2% sodium succinate medium (pH 6.5—7.0) at 32℃ for 72 h, about 5—8 units of benzoate 1,2-dioxygenase per gram cell was obtained.更多还原