【摘要】 本文介绍了不浓缩尿液本周氏蛋白电泳银染色法,对42例不同蛋白浓度的尿液标本、稀释血清标本进行了检测,并与考马司亮兰染色法对照。结果表明银染色法尿蛋白含量仅为0.05 g/L的正常浓度,稀释血清蛋白含量至1.5mg/L时尚能显现电泳带,而考马司亮兰染色仅尿蛋白浓度为0.2g/L和烯释血清蛋白至78mg/L时能显电泳带。本文还对电泳方法学及银染色的某些影响因素进行了讨论。更多还原

【Abstract】 Presented in this paper is a report of silver stain method following electrophoresis for detecting Bence Jones proteins in unconcentrated urine. 42 subjects with different concentrations of proteins in urine and diluted sera have been evaluated with this method in comparison with Coomassie Brilliant Blue stain. Our results show that the protein belts can be seen in the urine and diluted sera at the concentration of 0.05 mg/L and 1.5 mg/L respectively. In Coomassie Brilliant Blue stain the belts can only be observed when the concentration of protein in urine is 0.2g/L and when the level of proteins of diluted sera is 78mg/L. Besides, the method for electrophoresis and the factors affecting silver stain are also discussed.更多还原