【摘要】 含脊髓灰质炎病毒1型(简称 PV1)全基因7.4Kb cDNA 和编码 PV1外壳蛋白 VP12.5Kb 的 cDNA 片段的两株重组痘苗病毒(RV-1和 RV-2)表达了 PV1抗原。经荧光标记的抗 PV1型特异性单克隆抗体检测,感染了 RV-1和 RV-2的 Herp-2细胞呈阳性荧光反应。免疫印迹实验证实两株重组痘苗病毒分别表达 PV1抗原三条和两条特异性蛋白带。免疫家兔后能诱导产生抗 PV1中和抗体。本文对重组病毒表达 PV1抗原的效率进行了初步讨论。更多还原

【Abstract】 Two recombinant vaccinia virus(RV-1 and RV-2)strains containing a full genome of polio virus(PV)type Ⅰ and encoding structural protein Vpl 2.5 Kd cDNA of PV have successfully expressed the antigenic protein of PV type Ⅰ.The antigen was located in the infected Hep-2 cell with an appearence specific granular particles by IF assay.The expressed PV proteins showed as four bands in RV-1 and two bands in RV-2 on Western Blot.They reacted well with anti-PV McAb.The animals immunlized with the two recombinant virus strain could produce neutralization antibodies against PV type Ⅰ.This paper also presented the preliminary results of expression level of PV type Ⅰ anti- gen of recombinant virus更多还原