Some important factors which influence the frequency inducing pollen plants were researched. The materiels for experiment were F1 hybrids growing in greenhouse.The results showed that differentiation frequency of calli was increased significantly when BA or BA. + KT were added into the inducing media, that inducing frequency of calli was 66.7% higher when 0.17M sucrose and 0.17M glucose were added than when 0.26M sucrose were added, that green plantlet frequency was increased strikingly when calli were incu...
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