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茄子花药培养及单倍体育种
In-Vitro Anther Culture and Haploid Breeding of Solanum melongena
【摘要】 取花粉发育为单核中期的茄子(Solanum melongena Uar.Serpentinum Bailey)花药进行离体培养。以Co?对茄子进行花药予处理,提高愈伤组织诱导频率。对花药采用30℃恒温培养一用,然后降至17~28℃,加速花粉起动。通过愈伤组织及胚状体的途径获得花粉植株。观察了其后代分离及稳定,并通过田间鉴定及筛选,培育出抗病、高产、质佳的茄子新品系86-1。已开始应用于生产。
【Abstract】 Culture of eggplant (Solanum melongena var. Serpentinum Bailey) anthers containing raicrospores at mid uninucleate stage was made in vitro. To increase inductive frequency of the calli, the anthers were pre-treated with Co60.The anthers were cultured under 30℃. After one week, the temperature fell to room temperature. The pollen plants were obtained from the callus and embryoid. The segregation and stability of the descendants of the pollen plants were observed. By identifying and screening in field, a new disease-resistance line 86-1 with high yield and good quality has been released. And it has been gotten into production.
【Key words】 Pollen plant; Haploid; Induction; Segregation; Identifying and screening; Variation;
- 【文献出处】 生物技术 ,Biotechnology , 编辑部邮箱 ,1991年05期
- 【被引频次】20
- 【下载频次】211