【摘要】 本文利用Whitten′s液作为贮存液,采用台盼蓝染色和37℃培养两种方法研究小鼠各期胚胎6℃保存情况。37℃培养结果表明,小鼠胚胎在6℃保存是可行的;8—细胞以后的各期胚胎的贮后存活率高于8—细胞以前各期。台盼蓝染色鉴定结果一般偏高。未经冷处理的对照组胚胎的37℃培养存活率达85—100％。更多还原

【Abstract】 Whitten’s culture medium was used in tne experiment of cold storage of mouse embryos from 1-cell stage to morula.The vitality and developmental potential of the embryos following cold storage were tested with two methods. One was typan blue staining and another was observing blastocyst expansion buring cultured at 37℃。The results indicated that it was aractical to store mouse embryos at 6℃. The survival rates of the embryos at and after 8-cell stage were higher than that of embryos before 8-cell stage.cold storage could bring the survival rates down and the longer the embryos storer the lower the survival rates were. The blastocyst exaansion rates of the embryos developed in control were 85-100%The experiment showed that Whitten’s medium was benificial to the culture of mouse embryos.更多还原