【摘要】 为了解决无籽西瓜育种中存在的种子发芽率低,成苗率低和无籽率低的难题,我们利用无籽西瓜茎尖进行组织培养.采用了正交旋转设计方法,筛选出了较适宜的分化培养基（NH₄NO₃500mg/1、KNO₂500mg/1、KH₂PO₄370mg/1、铁盐10ml,附加BA 0.5mg/1、IBA 0.4mg/1其余用量同MS基,pH=6.4）和生根培养基（在分化培养基的基础上去掉BA,糖为20g/1,适用于85—4品种。然后在此基础上,KNO₂增加的750mg/1,KT0.1mg/1即适用于85—1、85—3两个品种）分化率平均为3.6倍;生根事率为100％。研究结果证明:1.促进试管苗分化生长是由多因素多水平综合效应的结果,所以在筛选培养基时要注意多因素多水平的协调配比。2.尽管是相同种类的植物,由于品种间的差异大,对培养基的适应性也有差异。更多还原

【Abstract】 In order to solve the problem of low germination value,low seedling survive rate and low seedoess rate of seedless watermelon, We used stem-tip to propagate seedless watermelon by tissue culure technigue. Suitable differentiation medium (NH4NO3 500mg/l, KNO3 500mg/l, KH2PO4 370 mg/l,ferrite salt 10ml, BA 0.5mg/l and IBA 0. 4mg/l were complmented with,the applied quantities of others are the same with MS medium, pH=6. 4)and rooting medium (based on the differentiation medium)BA was removed,and add sucrose 20g/l,which is suitable for the variety 85-4. Based on it, the applied quantity of KNO3 was increased to 750mg/l and KT0.1mg/1 was added, which is suitable for the varieties 85-1 and 85-3. )were selected by perpendicular rotating design method. The differentiation rote is 3. 6 times and the rooting rate is 100%. The research results certificate. 1. It is a sythetical effection of different factors and levels that promote the differentiation and growth of the test-tube plantlets,therefore,we should pay attention to the coordination of different factors and levels when we select the culture medium. 2. The different adaptabilities to the medium are found because of great difference among the varieties even if the plants are in the same spiece.更多还原