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人脑胶质细胞瘤核染色质非组蛋白的聚丙烯酰胺凝胶电泳分析

Polyacrylamide Slab Gel Electrophoresis of Human Brain Glioma Nonhistone Chromatin Protein (NHCP)

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【作者】 王瑞珍

【Author】 Wang, Rui-zhen(Department of Biochemistry. Jiangxi Medical college,Nanchang 330006)

【机构】 江西医学院生化教研室 南昌 330006

【摘要】 用不连续SDS-聚丙烯酰胺凝胶电泳分析了人脑胶质细胞瘤与正常脑细胞核NHCP电泳图谱。从二者的NHCP电泳结果表明,脑胶质细胞瘤增加了一条表观分子量为3万的蛋白区带;表观分子量为1.75万~6.5万的蛋白区带染色明显加深。染色质紫外吸收光谱也有明显差异。总之,脑胶质细胞瘤核NHCP发生质与量的变化。

【Abstract】 The electrophoretogram of brain nonhistone chromatin protein (NHCP)from human brain glioma and normal human brain were analyzed by SDS polyacrylamide slab gel electrophoreses. Analytical results showed that a new NHCP band (apprpx. 30000mol. wt. ) was found in human brain glioma, other regions (approx, 17500-65000mol. wt. ) were deeply stained indication marked increase in human brain glioma. In general,NHCP of brain nucleus of human glioma was changed both in quantity and in quality.

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