【摘要】 应用抗HBV-X基因区编码蛋白的单克隆抗体,经免疫印迹技术和免疫组化法对30侧慢性乙肝患者肝内 HBxAg 存在状况的研究。结果表明 HBxAg 阳性率高低与肝组织的慢性化程度一致:CPH 为14.3%,CAH26.7%,CAH+LC50%。在血清抗-HBe 阳性者中阳性率为50%,高于 HBeAg阳性者(21.4%)或 HBsAg 伴抗-HBc 阳性者(25%)。本文提示抗-HBx 的存在可能与 HBVDNA的整合有关。更多还原

【Abstract】 A study on HBV-X gone in liver tissues of patients with chronic hepatis B,was per-formed with the method of immunoblot and immunohistochemical staining with monoclonalHBx antibody.Nine out of 30 patients with chronic hepatitis B were positive for HBxAg(30%).The positive rates of HBxAg were different in various pathological changes; chro-nic persistant hepatitis 14.3%(1/7),chronic active hepatitis 26.7%(4/15)and chronic ac-tive hepatitis+liver chirrhosis 50%(4/8)in patients with positive anti-HBe.While inpatients with positive HBeAg or those negative for emarkers,the percentages of HBxAg werelow,being 21.4%(3/14)and 25%(2/8)respectively.The expression of positive HBxAgwas compatible with the degree of pathological changes.The presence of HBxAg and highpositivity in anti-HBe positive patients suggested that the expression of HBxAg might berelated to the integration of HBV DNA in liver cells.更多还原