【摘要】 本文利用反相高效液相色谱法（RP-HPLC）对双氯芬酸钠（DS）血浓度的测定进行了研究,建立了灵敏、精确、简便、重现性好的分析方法。适用于药代动力学的研究。条件是在0.2mol/L NaAc-HAc缓冲溶液（pH5.0）中以乙醚-二氯甲烷3:1（V/V）提取。固定相为YWG C₁₈H₃₇,流动相为MeOH-0.02mol/L HAc（pH 5.5）65:35（V/V）、卡马西平（CZ）作内标,UV 280nm检测。线性范围0.24～22.8μg/ml,γ=0.999 9。最低检测浓度为60ng/ml。提取回收率为95.83％～97.58％。方法回收率为100.33％～106.46％。天内及天间精密度（GV）各为0.55％～1.11％及1.40％～2.91％。更多还原

【Abstract】 A simple, sensitive and reproducible RP-HPLC method was developed for the determiantion of diclofenac sodium (DS) in plasma. The samples were extracted by ether-dichloromethane in a pH5.0 HWc-NaAc buffer solution. A Gilson HPLC instrument, fitted with a YWG C8H17 10μm column (150×4.6mm) and a variable wavelength UV detector adjusted to 280nm, was used. The mobile phase contained of a mixture of MeOH and 0.02mol/L HAc 65:35(F/F). The flow rate was 1.0ml/min. Carbamazipine (CZ) was used as an internal standard. A linear calibration curve for DS was measured within a range of 0.24μg 22.8μg/ml. The limit of detection was 3.6ng and the lower concentration for detection was 60ng/ ml. Good coefficient of correlation (Y=-33.75+792.24X, r =0.999 9) was obtained, and an the average CV% of within and betwean-days were 0.88% and 2.35% respectively (n= 5). The anti-inflammatory agents for the routine analysis of large numbers of plasma samples usually obtained in pharmacokinetic studies were suitable.更多还原