The purpose of this study was to find the best effect's factor and potence of ferment by adding different types of surfactants for increasing the cell membran permeability of L-proline precursor L-glutamio aoid.The ammonium salt of aliphatic phosphornate ester was preferable for extra-direct precursor L-glutamic acid permeate into the cell to promoting L-proline biosynthesis; when it's concentration is 0.045%.It's amount of product was increased about 30% than control.
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