【摘要】 本试验用胰蛋白酶消化繁殖期公鸡睾丸。用 RPMI-1640 培养液制成细胞悬液.按离体细胞培养和 RIA 分析法研究锰对睾丸细胞分泌睾酮的影响。结果表明:离体培养时睾丸细胞分泌睾酮含量(ng/9.7×10~5细胞·ml·6h)对照组(0μmol Mn)为4.266±0.736.5μmol Mn组为5.394±0.154.100μmol Mn 组为5.421±0.099.500μmol Mn 组为4.511±1.031.1000μmolMn 组为3.957±0.731。结果提示:睾丸是锰对生殖内分泌系统的直接作用部位,一定浓度的锰可以促进睾丸细胞对睾酮的分泌功能.而高剂量的锰则有抑制作用。更多还原

【Abstract】 The testis cells were obtained from stud-cocks by pre-treatment with typsin and suspensions of testis cells were made by RPMI-1640 media.The effect of Manganese on testosterone secretion of testis cells was studied by cell culture and radioimmunoassay.The results showed that the testosterone secretion levels of testis cell in Control Group,50μmol Mn Group,100μmol Mn Group,500μmol Mn Group and 1000μmol Mn Group were 4.266±0.736,5.394±0.154,5.421±0.099, 4.511±1.031 and 3.957±0.731 ng/9.7×10~5cells·ml·6h respectively.The results suggested that the testis was one of the reproductive endocrine system influ- enced by Manganese and that adaptable Manganese level could stimulated testis cells to secrete testosterone but excess Manganese had inhibitory effect on tes- tosterone secretion of testis cells.更多还原