【摘要】 用 IE、SDS-PAGE 和 Western blot 对肺吸虫成虫抗原(PwA)成分进行分析.结果 IE 出现2条粗而明显的沉淀带。SDS-PAGE 显示29条蛋白带(分子量范围为90.0～6.0 kD),其中有10条主带,分子量分别为57、53、49.5、46、42、30、27、25、14和13 kD.经 Western blot 试验显示,肺吸虫病人血清(抗体)与 PwA 应答,可出现3条蛋白带,分子量分别为25、13和8 kD。更多还原

【Abstract】 The components of soluble antigen of Paragonimus vestermani(PwA)were analysed with immuno-electrophoresis(IE),Sodium dodecyl sulfate-polyacrylamide gel eleetrophoresis (SDS-PAGE),and western blot.Two broad and distinct bands only were detected in IE. 29 protein bands were found to be present when adult worm homogenates were subjected to SDS-PAGE analysis,and their molecular weights ranged from 90.0～6.0 kD.The molecu- lar weight of the 10 most prominent protein bands was corresponeded to 57,53,49.5,46, 42,30,27,25,14 and 13 kD respectively.The western blot examination of PwA with sera of proven cases of paragonimiasis revealed 3 bands,whose protein molecular weights were 25, 13,and 8 kD respectively.更多还原