【摘要】 通过大量培养克隆有 HPV11、16、18、51型 DNA 序列的菌株,扩增其质粒,并采用碱变性法提取、纯化质粒 DNA,然后对质粒进行酶切、电泳回收 HPV DNA 片段并进行生物素标记,制备出生物素标记的 HPV11、16、18、51型 DNA 探针.用斑点杂交方法检测探针的灵敏度为 HPV11、16型0.05pg,HPV18 0.25 pg,HPV51 0.1 pg.更多还原

【Abstract】 Biotin-labeled DHA probes of human papillomavirus 11,16,18,51 types are produced by the following procedures.Fitst,culture of the strains of E.Coli DH-52 which contain cloned put-18 plasmid and plasmid amplification.Second,extraction and purification of the plasmids after alkaline denaturation.Third,enzyme digestion of the plasmids.Fourth,bi- otin-labeling of HPV 11,16,18,51 DNA probes.The sensitivity of HPV 11,16,18,51 DNA probes.is detected by using dot hybridization.The results is that it is 0.05 pg for HPV 11,16,0. 25 pg for HPV 18,0.1 pg for HPV 51.更多还原