【摘要】 为了进一步证实原位鸡胚水晶体是一种有用的实验系统,我们利用相同的实验处理对原位和离体培养水晶体的形态学进行了比较研究。实验试剂用单克隆抗体NC6及促癌剂TPA,两者均已证明可促使水晶体增大。20期的鸡胚作为实验材料。在原位实验:NC6或TPA被注射到右眼内,未注射的左眼作为对照;在离体实验:实验组水晶体培养于含有NC6或TPA的培养液,对照水晶体则培养于不含NC6或TPA的培养液。处理24小时后,测定水晶体大小并观察它们的形态结构。原位水晶体实验结果表明,实验组水晶体明显大于对照组(P<0.01),但其形态结构在两组之间未发现任何可见性变化。离体培养水晶体的实验结果与此相同。众所周知,离体水晶体的发育不受胚胎体内任何机制的影响。原位水晶体实验结果与离体实验相同这一事实进一步证明了原位鸡胚水晶体是一种有用的实验系统。这个系统看来十分适用于进行生长控制、化学物质对发育的影响等重要问题的详细分析。更多还原

【Abstract】 In order to confirm that the chicken embryonic lens in vivo is a useful experimental system, we carried out a comparative study on morphology of chicken embryonic lenses in vivo and in vitro with the same experimental treatment. Monoclonal antibody NC6 and tumor promoter TPA (12-tetradecanoyl phorbol-13-acetate), which have been proved to make lenses bigger, were used as the experimental agents, and chicken embryos at stage 20 were used as the experimental materials. For the experiment of lenses in vivo, NC6 or TPA was injected into right eyes while uninjected left eyes served as the control. For that in vitro, lenses of the experimental group were cultured in the media with NC6 or TPA. and those of the control group in the media without NC6 or TPA. The size of lenses was measured and their structure was observed after the treatment for 24 hours. The results of lenses in vivo indicated that the size of the lenses from the experimental group is obviously larger than that from the control group (P<0.01), but no visible change in their structure could be found in the experimental group. The experiment of lenses in vitro shows the same results. It is well known that the development of lenses in vitro is not subjected to effects of any mechanism within embryos. The fact that the result of lenses in vivo is the same as that in vitro confirms that chicken embryonic lens in vivo is a useful experimental system. This system seems to be very suitable for a detailed analysis of some important questions, such as the growth control, effects of chemicals on development etc. The project was supported by Grants from NSF of China and SM of Jiangsu Province.更多还原