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重金属对植物离体组织和细胞生长分化的影响

The Effects of Heavy Metals on Growth and Differentiation of Plant Cell and Tissue in Vitro.

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【作者】 蒋婉茹杨居荣许嘉琳王素芬

【Author】 Jiang Wanru. (The Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences)

【机构】 中国农业科学院作物研究所北京师范大学环境科学研究所北京师范大学环境科学研究所 北京 100081北京 100088北京 100088

【摘要】 试验采用组织培养技术,以小麦未成熟胚、烟叶组织及胡萝卜无性细胞系为材料,研究其在含不同浓度的重金属培养基中生长和分化的影响。结果发现:烟叶组织对几种重金属的富集能力不同,其顺序为Cu2+>Cd2+>As3+>As5+>Pb2+,而它们对于烟叶组织的毒性效应依次为As3+>As5+>Cu2+>Cd2+>Pb2+。由此看出,对离体组织毒害的强弱并不完全取决于累积数量。

【Abstract】 In order to conduct further research on the mechanism of the poisons of heavy metals (Cu, As, Cd, Pb)to plant tissue and plant cell, tissue culture method was used in the experiment. Immature embryos of wheat, leaf tissue of tobacco and somaclonal cell of carrot were incubated in the media containing various concentrations of heavy metals. It has been found that the accumulative amounts of heavy metals in the leaf tissue of tobacco is different. The order of accumulative amount is as following, Cu2+>Cd2+>As3+>As5+>Pb2+. But the order of poisonous effect to leaf tissue in tobacco is As3+>As5+>Cu2+>Cd2+>Pb2+. These facts indicate that the poisonous extent does not depend on the accumulative amounts of heavy metals.In addition, restorative ability or plant tissue sue which was suffered in heavy metals poisonhas also been preliminarily tested.

【基金】 国家自然科学基金
  • 【文献出处】 农业环境科学学报 ,Journal of Agro-environmental Science , 编辑部邮箱 ,1991年05期
  • 【被引频次】10
  • 【下载频次】237
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