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Ri质粒在绿豆离体器官培养中转导发根的全息现象

THE HOLOGRAPHIC PHENOMENON OF HAIRY-ROOTS TRANSFORMED BY Rl PLASMID IN EXPLANT CULTURE OF PHASEOLUS RADIATUS L

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【作者】 倪德祥; 李建兵;

【Author】 Ni Dexiang Li Jianbing( Department of Environment & Resources Biology, Fudan University, Shanghai, 200433, PRC )

【机构】 复旦大学环境和资源生物学系; 复旦大学环境和资源生物学系 上海200433; 上海200433;

【摘要】 生物体每个相对独立部分的结构和功能,都是其整体结构和功能的成比例的缩小,这种现象称为生物全息律.以绿豆(Phaseolus radiatus L.)的上下胚轴和叶片为外植体,进行了Ri质粒转化根的试验.结果表明,离体的绿豆上下胚轴和叶片,在进行发根培养时,同样存在着生物全息现象.这种现象为研究离体器官的形态发生,以及发根农杆菌Ri质粒的发根效应,提供了一种新的观点.

【Abstract】 The structure and function of each independent part in the organism were proportionably reduced for the whole. The phenomenon was called the bio-holographic law.The explant culture of embryonic axis and blade of Phaseolus radiatus L. in vitro was induced to produce hairy-roots by Ri plasmid. The experiment showed that hairy-roots transduction of embryonic axis and blade in vitro was according with the bio-holographic law. The bio-holographic phenomenon provides a novel viewpoint for studing morphogenesis of isolated tissues and transformation effect of Agrobacterium rhizogenes Ri plasmid.

【基金】 植物学博士点基金资助项目
  • 【被引频次】7
  • 【下载频次】33
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