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白细胞介素2和黄芪多糖对小鼠NK细胞作用的细胞化学和超微结构观察
EFFECTS OF INTERLEUKIN-2 AND POLYSACCHARIDE OF ASTRAGALUS ON MURINE NK CELL: CYTOCHEMISTRY AND ULTRASTRUCTURE
【摘要】 本文报道在白细胞介素2(IL-2)及黄芪多糖(PA)作用下,小鼠NK细胞的细胞化学和超微结构变化。实验表明,NK细胞酸性磷酸酶、酸性醋酸酯酶及氯醋酸AS-D酯酶均为阳性。经IL-2和PA的处理,前两种酶的活性增加,阳性细胞百分率增高,并与NK活性相关。IL-2培养后的NK细胞超微结构表现为胞体变大,表面不规则,胞质丰富,细胞器增多等特点。
【Abstract】 In this paper, murine natural killer (NK) cells were studied with cytochemistry and electron-microscopy after treated with interleukin-2 (IL-2) and polysaccharide of astragalus (PA). The results showed that acid phosphatase (AcP), acid naphthylacetate esterase (ANAE) and naphthol-AS-D chloroacetate esterase (NCAE) were positive in NK cells, and the activity of AcP and ANAE was enhanced by IL-2 and PA. Significant changes of NK cells in ultrastructure took place after preculturing with IL-2.
【关键词】 白细胞介素2;
黄芪多糖;
酸性磷酸酶;
酸性醋酸酯酶;
氯醋酸AS-D酯酶;
细胞化学;
超微结构;
【Key words】 Interleukin-2 (IL-2); Polysaccharide of astragalus (PA); Acid phosphatase (AcP); Acid naphthyl acetate esterase (ANAE); Cytochemistry; Ultrastructure;
【Key words】 Interleukin-2 (IL-2); Polysaccharide of astragalus (PA); Acid phosphatase (AcP); Acid naphthyl acetate esterase (ANAE); Cytochemistry; Ultrastructure;
【基金】 国家自然科学基金
- 【文献出处】 解剖学报 ,Acta Anatomica Sinica , 编辑部邮箱 ,1991年01期
- 【被引频次】19
- 【下载频次】47