STUDIES ON INTRODUCTION OF FOREIGN GENES INTO CULTURED CELLS OF Oryza sativa INDICA USINC Agrobacterium Ti PLASMID SYSTEM

【摘要】 <正> Either bacterial attachment or cellulose fibrillar elaboration was hardly observedduring the cocultivation of the cultured suspension cells of Oryza sativa Indica with thestrain C58C1 Rif~r of Agrobacterium tumefaciens that was not specially pretreated. Onthe other hand, quite a lot of Agrobacterium cells were found to adhere to the surface ofcultured rice cells and a number of cellulose fibrils were produced around the specifiedbacteria when phenolics-pretreated bacteria were cocultivated with rice suspension cellsin common culture media, especially in complex culture solutions. The complex culturesolution was the bacterium-free filtrate of hormone-containing MS medium which hadbeen utilized to incubate carrot cells and the newly wounded hypocotyl segments fromtomato and Agrobacterium cells. Detecting experiments demonstrated that both NPT Ⅱ andNOS genes, located on the T-DNA segment of chimaeric plasmid pGV3850 :: 1103neo, weretransferred and expressed in the cultured cells of O. sativa Indica in the更多还原

【Abstract】 Either bacterial attachment or cellulose fibrillar elaboration was hardly observedduring the cocultivation of the cultured suspension cells of Oryza sativa Indica with thestrain C58C1 Rif~r of Agrobacterium tumefaciens that was not specially pretreated. Onthe other hand, quite a lot of Agrobacterium cells were found to adhere to the surface ofcultured rice cells and a number of cellulose fibrils were produced around the specifiedbacteria when phenolics-pretreated bacteria were cocultivated with rice suspension cellsin common culture media, especially in complex culture solutions. The complex culturesolution was the bacterium-free filtrate of hormone-containing MS medium which hadbeen utilized to incubate carrot cells and the newly wounded hypocotyl segments fromtomato and Agrobacterium cells. Detecting experiments demonstrated that both NPT Ⅱ andNOS genes, located on the T-DNA segment of chimaeric plasmid pGV3850 :: 1103neo, weretransferred and expressed in the cultured cells of O. sativa Indica in the optimum trans-formation condition of the present experiment. Integration of the foreign genes into riceDNA was confirmed by Southern blot hybridization.更多还原