【摘要】 通过在匀浆缓冲浪中添加抗坏血酸并结合蔗糖密度梯度离心和差速离心法等分离纯化了水稻叶绿体 DNA(ctDNA),得率高达100μg/100g叶,其纯度足以用于限制酶分析和分子生物学研究.环状水稻ctDNA总长度为129.5Kbp,PvuⅡ、SalⅠ、PstⅡ、Hind Ⅲ、EcoRⅠ和BamHⅠ在ctDNA上的切点分别为11、12、17、37、67和44.更多还原

【Abstract】 By homogenizing rice leaves in a buffer solution containing ascorbic acid and by centrifugalizing the suspension repeatedly the intact chloroplast was isolated, and the chloroplast DNA was finally obtained. The ctDNA is in high yield ( about 100 μg DNA/100 g leaves ) and pure enough for restriction endonuclease analysis and other purpose of molecular biology research.The total length of the circular rice ctDNA is 129. 5Kbp. On the ct-DNA, PvuⅡ. SalⅠ. Pst Ⅰ. Hind Ⅲ. EcoR Ⅰ and BamH Ⅰ has 11, 12, 17, 37, 67, and 44 cleavage sites respectively.更多还原