【摘要】 本文研究了三组大鼠(对照组、缺铁组、补铁组)中性粒细胞吞噬杀菌功能。三组中性粒细胞化学发光峰值、5分钟积分面积值均有显著性差异,缺铁组分别为对照组的41％及32％,示中性粒细胞NADPH氧化酶活性降低,吞噬伴随的“呼吸爆发”功能受损。髓过氧化物酶活性三组间也有显著性差异,缺铁组为对照组的30％,示中性粒细胞杀菌功能障碍。补铁一周后,补铁组上述三项指标明显恢复,分别达正常组值的79％、76％及66％,与缺铁组相比有显著性差异,但尚未达正常水平。若按其每日平均恢复速率,推算出补铁后中性粒细胞吞噬伴随的“呼吸爆发”功能完全恢复约需11天,髓过氧化物酶活性完全恢复约需14天。更多还原

【Abstract】 Studies were made to deter-mine the neutrophil’s phagocytosis andbactericidal function in three groups ofrats (control, iron deficiency, and ironsupplement). Results showed that therewere significant differences in values ofchemiluminescence (CL) among threegroups. The values of peak CL and flveminutes integrated CL were markedly de-creased in noutrophils of iron-deficientrats, accounting for only 41% and 32% of thecontrol’s values respectively. These sug-gested that the activity of NADPH oxidasewas decreased, and the function of respira-tory burst of neutrophils was impairedThe activity of myeloperoxidase in theiron-deficient neutrophils was also signi-ficantly lower than that in the controlcells. It constituted only 30% of the con-trol’s value, indicating that the bactcrici-dal function of neutrophils was injured.One week after iron administration, thelow values of the peak CL, the five mi-nutes integrated CL and the activity ofmyeloperoxidase all went up apparently,but not reached the normal levels yet.Thetime the function of neutrophils in iron-deficient rats returned to normal may berelated to the process of neutrophil matu-ration in bone marrow.更多还原