【摘要】 抗人外周血T淋巴细胞单克隆抗体（McAb）SOKT₁通过SPDP与蓖麻蛋白A链（RTA）交联制成免毒素SOKT₁-RTA,在20mmol/L氯化铵存在条件下,能够杀伤82%人外周血T细胞,并使其³H-亮氨酸掺入下降为对照的6.2%。SOKT₁-RTA的作用能够被未结合RTA的SOKT₁部分阻滞,而另一无关McAb末显示阻滞作用。表明:SOKT₁-RTA结合物具有SOKT₁的抗原结合特异性和RTA的细胞毒性,有可能成为清除人骨髓中T细胞的有效制剂。更多还原

【Abstract】 An immunotoxin, SOKT₁-RTA, was prepared successfully by conju-gating a murine anti-human T lymphocytemonoclonal atibody （McAb） SOKT₁ andthe ricin toxin A chain （RTA） with theheterobifunctional linker SPDP. At thepresence of 20mmol/L NH.Cl, theSOKT₁-RTA conjugate killed 82% of thehuman peripheral blood T cells（PBT）,and decreased their ³H-Leu incorporationto 6.2% of the untreated controls. Thetoxic effect of the immunotoxin can bepertially blocked by unconjugated SOKT₁but not by an irrelevant McAb D_?1. Theseresults indicated that the SOKT₁-RTAconjugate still retains both functions ofits component parts:binding to human PBTspecifically, and inhibiting protein syn-thesis. It may be an effective reagent forin vitro depletion of immunocompetentT cells from human bone marrows.更多还原