【摘要】 进行了5′—AMP—QT₄亲和层析柱纯化乳酸脱氢酶（LDH）的试验。采用该方法一步即可从猪骨骼肌组织粗提液中获得高比活（566u/mg）的乳酸脱氢酶,酶活性回收率达85％以上。更多还原

【Abstract】 An affinity column based on 5’-AMP-QT₄ was applied to purify lactate dehydrogenase （LDH）. Using this method, LDH with high specific activity （566 u/rag）has been isolated from crude extract of porcine skeletal muscle by a single step. The rate of recovery of LDH activity was more than 85％.更多还原