【摘要】 根据广州市蟑螂种群生态分布,应用葡聚糖凝胶层析、SDS-PAGE等生化技术,以及皮内试验、特异性IgE检测等免疫学方法,对美洲大蠊、澳洲大蠊和德国小蠊等五种蟑螂浸液,作了化学特性及免疫学活性的研究。结果显示:1、美洲大蠊若虫是本地区蟑螂过敏的主要致敏种群,其活性组分为F₂;分子量范围64～79KD。2.美若浸液免疫活性与其蛋白质的分子量有关,通过葡聚糖凝胶层析,可去除无活性的组分,以达到纯化的目的。更多还原

【Abstract】 According to the ecological distribution of Cockroach species in Guangzhou, five kinds of Cockroach extracts (i. e. Periplaneta americana, Periplanta australasiae, Blatteria germanica and so on) were studied on chemo-immunity by sephadex gel chromatography, SDS-PAGE, intradermal test and detection of SIgE. The results showed that: (1) Nymph of periplaneta americana was the most important allergic species in this area; its active part was in F2 with Mol. wt. of 64-79 KD. (2) The immuno-active of extract was related to its protein molecular weight and the non-active portions could be removed by SGC.更多还原