【摘要】 用控制孔玻璃珠(CPG)填充的色谱柱,以水和盐的水溶液为淋洗液,研究了具有聚电解质性质的腐植酸(HA和FA)的体积排阻色谱分级。以水为淋洗液时,HA和FA均在空体积处被淋洗出来。证明了这种异常排阻现象是由CPG表面的负电荷和HA及FA上的负电荷之间的静电排斥作用引起的。用盐的水溶液为淋洗液时,静电排斥作用得到抑制,并且不会引起吸附。盐溶液浓度不同,淋出曲线有所差异。讨论了盐浓度对聚电解质体积排阻色谱的影响。更多还原

【Abstract】 The size exclusion chromatograms of humic acid(HA) and fulvic acid(FA) were studied by using column packed with controlled-pore glass(CPG), and with water or aqueous salt solution as eluents. The experiment shows that an unusual exclusion phenomenon was verified to be the coulombic repulsive force induced by CPG and HA or FA. This unusual exclusion could be inhibited by using aqueous salt solution as eluent, and the concentration of salts in the eluent has a marked effect on the elution chromatograms. The influence of the concentration of added salt on the size exclusion chromatograms of polyelectrolyte was discussed.更多还原