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人类精子超低温冻存技术和临床应用研究

Study on Cryopreservation Technique of Human Semen and its Clinical Applications

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【作者】 陈奕权王建新华庆和余庆亮刘秀玲冯殿黄秀兰

【Author】 Chen Yiquan Wang Jianxin Hua Qinghe (Department of Histology and Embrylogy,Fujian Medical College,Fuzhou)

【机构】 福建医学院组织学与胚胎学教研室福建医学院组织学与胚胎学教研室福建医学院计划生育研究室

【摘要】 本文对176份人精液标本采用以甘油和蛋黄为主要成分的冷冻保护剂,安瓶储存,逐级降温保存精子。冻存期从24h 至3年,冻存率达72%。快速复温可使精子复活并用于110对男性不育夫妇,供者精液人工授精(AID)成功53人次,成功率为48.2%。每次妊娠的平均治疗周期为1.84。已出生30个健康婴儿,流产率在非 AID 的自然流产率范围内。

【Abstract】 In this study, 176 semen speciums were mixed with the protective medium that contained mainly the glycerl and yolk,and were reserved in amples with the temperature degrating gradually. The cryop- reservation period of semen specimen lasted from 24 hours to three years. The average cryosurvival rate of the sperm was 72%. 110 cases of sterile women were inseminated with cryopreservation semen from the donors. Fifty—three pregnancies occurred in 98 treatment cycles. 48.2% conception resulted. Mean treatment cycle per conception was 1.84. Thirty normal and healthy babies were bred. There were 3 natural miscarriges.

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