【摘要】 分别采用丙酮沉淀、硫酸铵分步沉淀和柱层析技术从链霉菌85—39发酵液中分离、纯化出APH(3′)抑制剂85—39,并对其部分理化性质进行了测定。结果表明:抑制剂85—39为相对分子质量大于3000的水溶性多肽物质,对APH(3′)表现出强的抑制作用。该抑制剂经丙酮沉淀后的水抽提液对热、碱较稳定,对酸较不稳定,但经离子交换柱初步层析纯化后,其稳定性较差。更多还原

【Abstract】 The APH（3’） inhibitor 85—39 was isolated by precipitation with acetone from the broth filtrate and then extracted with distilled water. The active component was purified by column chromatography on CM-Sephadex C-50（NH₄⁺ form）.Some properties of the inhibitor 85—39 were given. It was found that the inhibitor 85—39 and related compounds are macromolecular polypeptides which are very unstable.更多还原