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普通小麦×栽培二棱大麦属间杂种及其回交后代选育和染色体的稳定性
INTERGENERIC HYBRID OF TRITICUM AESTIVUM WITH HORDEUM DISTIOHUM AND THE SELECTION AND CHROMOSOME STABILITY OF ITS BACKCROSS PROGENY
【摘要】 以小麦为母本与大麦杂交,通过幼胚培养,获得小大麦属间杂种。杂种自交不育。以中国春和昌农82小麦的混合花粉给杂种授粉,得到回交一代(BC1F1),结实率为4.35%。以78380—1和密穗早小麦的混合花粉及小偃107分别与 BC1F1回交,获得回交二代(BC2F1),结实率为45.45%和31.25%。F1体细胞染色体数为非整倍体,其变幅从18到27条之间,以2n=2l条较多。花粉母细胞减数分裂中期Ⅰ单价体细胞为37.0%,1—4个不等二价体细胞为63.0%。F1形态类似普通小麦。回交后代表型多为亲本中间型,也有少数超亲现象。在BC2F4代中,整倍体细胞(2n=42)占大多数(80.83%),染色体构型比较复杂,主要有21Ⅲ,20Ⅲ+2 Ⅰ和19Ⅲ十4 Ⅰ三种,以21Ⅲ的细胞较多(75.03%)。从分离后代中选育出单体(21Ⅲ+1 Ⅰ)和二体(22Ⅲ)异附加系及具突出性状的新类型。
【Abstract】 Intergenous F1 Hybrids between common wheat and cultivated barley were successfully obtained by means of immature embryo cul- ture.The hybrids were self-sterile.In the back cross,mixed pollen of Chinese Spring and Changnong 82 was used to pollinate the hybrids and the BC1F1 was obtained as a result.The seed set was 4.35%.Us- ing mixed potlens of 78380-1 and Misuizao,and the pollen of xiao- yah 107 to pollinate BC1F1 separately,the BC2F1 was produced. The seed set was 45.45% and 31.25% respectively.Somatic chromo- some numbers of F1 plant were aneuploid.They varied from 18 to 27 and 2n=21 in most cases.In the MI of PMC,univalent cells were 37.0% and bivalent cells ranging 1-4.were 63.0%.F1 plant were similar to commou wheat in morphological characters.The phenotype of backcrossed progenies was intermediate to their parent,with a few showing overparent appearance.In progenies of BC2F4,the euploid cell(2n=42)dominated.The chromosome configuration was compli- cated,but there were three major kinds of them,21Ⅱ, 20Ⅱ+2Ⅰ and 19Ⅱ+4Ⅰwith most cells being 21Ⅱ.Monosomic and disomic addition lines were selected from separate progenies including new ty- pes which have useful agronomical characters.
【Key words】 Common wheat; Cultivated twn-rowed barley; Immature embryo culture; Backcross progeny; Chromosome stability;
- 【文献出处】 西北植物学报 ,Acta Botanica Boreali-Occidentalia Sinica , 编辑部邮箱 ,1991年01期
- 【被引频次】12
- 【下载频次】41