【摘要】 本研究用自行提纯的GM₃观察了它对人单核样白血病细胞的促分化作用,以及促分化前后蛋白激酶C活性的影响。根据电镜观察、吞墨试验及NBT还原试验的结果,表明GM₃确能促使J6-2细胞分化;另外GM₃在促分化后,J6-2细胞的总PK-C活性下降。作者讨论了GM₃抑制蛋白激酶C的可能机理。更多还原

【Abstract】 Thee authors studied the PK-C activity of J6-2 cells before and after differentiation induced by ganglioside GM₃. By electron-microscopic observation, phagocytic capability and NBT reduction test, GM₃ was shown to induce the differentiation of J6-2, a leukemic cell line established from a Chinese monocytoid leukemic patient. After differentiation, the total PK-C activity of J6-2 cells decreased. The authors discussed the possible mechanism of GM₃’s action.更多还原